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AIM:To construct a recombinant strain which highlyexpresses catalase of Helicobacterpylori(H.pylori) and assaythe activity of H.pylori catalase.METHODS:The catalase DNA was amplified from H.pylorichromosomal DNA with PCR techniques and inserted intothe prokaryotie expression vector pET-22b (+),and thenwas transformed into the BL21 (DE3) E.coli strain whichexpressed catalase recombinant protein.The activity of H.pylori catalase was assayed by the Beers & Sizers.RESULTS:DNA sequence analysis showed that thesequence of catalase DNA was the same as GenBank’sresearch.The catalase recombinant protein amounted to24.4% of the total bacterial protein after induced with IPTGfor 3 hours at 37℃ and the activity of H.pylori catalasewas high in the BL21 (DE3) E.coli strain.CONCLUSION:A clone expressing high activity H.pyloricataiase is obtained,laying a good foundation for further studies.
AIM: To construct a recombinant strain which highlyexpresses catalase of Helicobacter pylori (H.pylori) and assay the activity of H.pylori catalase. METHODS: The catalase DNA was amplified from H.pylorichromosomal DNA with PCR techniques and insertedintotheprokaryotieexpression vector pET-22b (+), and then was transformed into BL21 (DE3) E. coli strain whichexpressed catalase recombinant protein. The activity of H. pylori catalase was assayed by the Beers & Sizers. RESULTS: DNA sequence analysis showed that thesequence of catalase DNA was the same as GenBank’sresearch. catalase recombinant proteinized to24.4% of the total bacterial protein after induced with IPTGfor 3 hours at 37 ° C and the activity of H.pylori catalasewas high in the BL21 (DE3) E.coli strain.CONCLUSION : A clone expressing high activity H. pyloricataiase is obtained, laying a good foundation for further studies.