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以二倍体、四倍体及经秋水仙素处理的黄花蒿植株为试材,采用整体透明技术制备黄花蒿叶片样品,对不同倍性植物的叶片气孔保卫细胞长轴长、短轴长及其周长进行考察,探讨气孔保卫细胞大小与植株倍性的相关性。结果表明:以10%氢氧化钾溶液为透明剂,透化时间为1.5h的透化效果最好;二倍体与四倍体植物的气孔保卫细胞长轴长、短轴长及其周长差异显著,其周长范围分别为57.19~70.33μm和83.36~91.99μm;利用二倍体与四倍体的周长分布在不同区间范围,可以对秋水仙素处理的黄花蒿植株进行快速的初步倍性鉴定。
The diploid, tetraploid and the colchicine-treated Artemisia annua plants were used as materials to prepare the leaves of Artemisia annua L. by using the whole transparent technique. The long and short axis length and the short axis length of the stomata guard cells of different ploidy plants The perimeter of the study to investigate the stomatal guard cell size and plant ploidy correlation. The results showed that the permeation time of 1.5h was best when 10% potassium hydroxide solution was used as the transparent agent. The long-axis, short-axis length and its perimeter of stomatal guard cells of diploid and tetraploid plants The difference was significant, and its perimeter range was 57.19 ~ 70.33μm and 83.36 ~ 91.99μm, respectively. Using the circumflex distribution of diploid and tetraploid in different interval range, the colchicine-treated A. annua plants could be rapidly and preliminarily Ploidy identification.