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在电镜标本制作时,一般采用戊二醛作为固定剂。戊二醛也是一种有效的杀菌剂,可杀灭多种微生物,包括细菌繁殖体、芽胞、病毒等。据报导戊二醛的作用浓度为2%,最适PH为7.5～8.5,作用时间对繁殖体需10～20分钟,对细菌芽胞需4～12小时。鉴于戊二醛具有杀菌和固定组织、细胞的双重作用,为微生物标本的电镜观察提供了有利
In the production of electron microscopy specimens, the general use of glutaraldehyde as a fixative. Glutaraldehyde is also an effective bactericide that can kill many kinds of microorganisms, including bacteria, spores, viruses and so on. It has been reported that the concentration of glutaraldehyde is 2%, the optimum pH is 7.5 ~ 8.5, the action time takes 10-20 minutes for propagules, and 4-12 hours for bacterial spores. In view of glutaraldehyde with bactericidal and fixed tissue, the dual role of cells for microbiological specimens of electron microscopy provides a favorable