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子宫内膜损伤是导致宫腔粘连的最主要原因,建立有效的子宫内膜损伤动物模型是研究此类疾病发生、发展和治疗反应等不可或缺的支撑条件。通过宫腔注射95%的乙醇制造大鼠(Rattus norvegicus)子宫内膜损伤模型,检测胚胎植入数目来分析子宫内膜损伤对大鼠生育情况的影响,观察大鼠子宫内膜厚度、腺体数量和纤维化面积,分析乙醇处理对子宫内膜的影响;检测细胞角蛋白(CK-19)和波性蛋白(Vimentin)的表达水平,分析上皮细胞和间质细胞的增殖分化及子宫内膜损伤程度。结果显示,正常组大鼠子宫比损伤组更为平滑且有韧性,与正常组相比,损伤组大鼠生育率显著降低(P<0.01),子宫内膜厚度变薄(P<0.01)、腺体数量显著减少(P<0.01),纤维化面积显著增大(P<0.01),CK-19和Vimentin表达量显著下调。结果提示已成功建立大鼠子宫内膜损伤动物模型。
Endometrial damage is the leading cause of intrauterine adhesions. Establishing an effective animal model of endometrial lesion is an indispensable condition for studying the occurrence, development and treatment response of such diseases. Rat models of endometrial injury were established by intrauterine injection of 95% ethanol, and the number of embryo implantation was detected to analyze the effect of endometrial damage on the reproductive status of rats. The thickness of endometrium, The number of fibrosis and the area of fibrosis, the influence of ethanol treatment on endometrium was analyzed. The expression of cytokeratin (CK-19) and vimentin were detected, and the proliferation and differentiation of epithelial cells and interstitial cells and endometrium degree of damage. The results showed that the uterus of rats in normal group was smoother and more resilient than that in injured group. Compared with normal group, the fertility rate of injured group was significantly decreased (P <0.01) and the thickness of endometrium was thinner (P <0.01) The number of glands decreased significantly (P <0.01), the area of fibrosis increased significantly (P <0.01), the expression of CK-19 and Vimentin decreased significantly. The results suggest that animal model of rat endometrium injury has been successfully established.