稻褐飞虱是危害水稻的主要虫害之一,近年来在我国南方稻作区大发生,对我国的粮食安全构成了严重威胁。选育和利用抗虫品种是控制稻褐飞虱为害的最经济、有效的方法之一。对来源于普通野生稻的5个抗稻褐飞虱(BPH)基因进行了分子标记开发,通过杂交、回交和分子标记辅助选择转育和聚合BPH抗性基因到杂交水稻亲本中,获得了与抗BPH基因bph25(t),bph26(t),bph22(t),bph23(t),Bph24(t)紧密或较紧密连锁的SSR分子标记,这些标记的选择正确率达到60%～90%。成功地转育和聚合3～5个BPH抗性基因到5个杂交水稻亲本获得抗性基因聚合系。对其中9个抗性基因聚合系进行遗传背景分析和性状调查表明,遗传背景回复率已达90%以上,苗期和成株期(即全生育期)表现对BPH高抗性,主要经济性状与受体亲本(轮回亲本)基本没有差异。这些抗性基因聚合系在抗BPH水稻品种培育中具有重要的利用价值。研究结果为最终培育出一系列对BPH具有高抗性和持久抗性的水稻品种提供了物质基础和技术支持。 Nilaparvata lugens (Nilaparvata lugens) is one of the major insect pests that endanger rice. In recent years, Nilaparvata lugens (Nilaparvata lugens) has occurred in rice growing area in southern China, posing a serious threat to China’s food security. Breeding and utilization of insect-resistant varieties is one of the most economical and effective ways to control brown planthopper (BPN) infestation. Five BPH genes from Oryza rufipogon (Oryza sativa L.) were developed by molecular marker, and the BPH resistance genes were transferred to the parents of hybrid rice through hybridization, backcrossing and molecular marker assisted selection. BPH genes were closely linked or closely linked to SSR markers bph25 (t), bph26 (t), bph22 (t), bph23 (t) and Bph24 (t). The accuracy of these markers was between 60% and 90%. Three to five BPH resistance genes were successfully transferred and aggregated to five hybrid rice parents to obtain resistance gene lines. The genetic background analysis and traits investigation of 9 resistance gene lines showed that the genetic background recovery rate reached more than 90%, the seedling stage and adult stage (ie the whole growth stage) showed high resistance to BPH, the main economic traits There is basically no difference between the recipient parent and the recipient parent. These resistance gene polymerases are of great value in the cultivation of anti-BPH rice varieties. The results provide the material basis and technical support for eventually creating a series of rice varieties with high resistance and long-lasting resistance to BPH.