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本实验应用脑缺血动物模型对脑缺血及再灌流期间〔Ca~2+〕i(胞浆游离钙)和ATP、ADP、AMP的变化及尼莫地平与东莨菪碱的影响进行了探讨。材料与方法采用104只沙鼠,雄性60只、雌性44只。月龄3个月左右,平均体重50g。随机将动物分为13组。1组为正常对照组,仅分离颈部血管。其余12组按缺血50分钟及缺血50分钟再灌流10分钟、60分钟和120分钟分为4个时间组。每个时间组又分对照组(C)、尼莫地平组(N)、东莨菪碱组(S)。C、N、S组分别在缺血前15分钟以生理盐水0.25ml、尼莫地平1mg/kg、东莨菪碱0.45mg/kg腹腔注射。沙鼠在氯胺酮(200mg/kg)腹腔麻醉下,夹闭双侧颈总动脉,阻断血流50分钟,造成沙鼠前脑缺血。松开血管夹恢复血流,设计再灌流时间分别为10分钟、60分钟和120分钟,即制成沙鼠脑缺血及再灌流动物模型。实验结束后,立即将沙鼠在冰玻片上断头取脑,分离前脑大脑皮层。
In this study, we investigated the changes of [Ca ~ 2 +] i (cytoplasmic free calcium), ATP, ADP, AMP and the effect of nimodipine and scopolamine during cerebral ischemia and reperfusion on cerebral ischemia in animal model. Materials and Methods 104 gerbils, 60 males and 44 females were used. About 3 months old, the average weight of 50g. Animals were randomly divided into 13 groups. Group 1 as a normal control group, only the separation of the neck blood vessels. The remaining 12 groups were reperfused for 10 min at ischemia of 50 min and ischemia at 50 min, and divided into 4 groups at 60 min and 120 min. Each time group is divided into control group (C), nimodipine group (N), scopolamine group (S). Rats in C, N and S groups were intraperitoneally injected with 0.25 ml of normal saline, 1 mg / kg of nimodipine and 0.45 mg of scopolamine respectively 15 minutes before ischemia. Gerbils were anesthetized with ketamine (200 mg / kg) and the bilateral common carotid arteries were occluded to block the blood flow for 50 minutes, resulting in forebrain ischemia in gerbils. Releasing the blood vessel clip to restore the blood flow, design reperfusion time were 10 minutes, 60 minutes and 120 minutes, that is made of gerbil cerebral ischemia and reperfusion animal model. After the experiment, gerbils were immediately decapitated on ice-cold glass slides and the forebrain cerebral cortex was isolated.