以硫酸为离子交联剂,制备了几丁聚糖生物粘附性微球.与硫酸盐作为离子交联剂相比,本方法制备的微球交联程度高,呈较规则的球形,表面致密,机械强度高,在人工模拟肠液中可以保存24h以上.微球的粒径是影响药物释放动力学的重要因素.运用四因素二次正交回归组合设计方法考察了几丁聚糖浓度、交联剂浓度、交联时间和转速对微球粒径的影响,得出了制备几丁聚糖微球粒径的回归模型方程.统计检验表明,其中几丁聚糖浓度和搅拌转速对微球粒径的影响最为显著. Using sulfuric acid as ionic crosslinking agent, chitosan bioadhesive microspheres were prepared.Compared with sulphate as ionic crosslinking agent, the microspheres prepared by this method had a high degree of cross-linking and had a regular spherical shape with the surface Dense, high mechanical strength, artificial simulated intestinal fluid can be stored for more than 24h.The particle size of microspheres is an important factor affecting the drug release kinetics.Using quadratic quadratic regression quadratic regression combined design method investigated chitosan concentration, Cross-linking agent concentration, crosslinking time and speed on the particle size of the microspheres, the regression model equation for the preparation of chitosan microspheres was obtained.Statistical tests showed that the chitosan concentration and stirring speed of micro The effect of ball size is the most significant.