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目的 研究胶质瘤多药耐药 (MDR)的发生机理及逆转方法。方法 建立了C6 /adrMDR细胞系 ,经RT PCR及免疫组化染色分别研究了mdr l基因及P 糖蛋白 (P gp)的表达 ;采用MTT药敏试验及HPLCA测定细胞内阿霉素浓度的方法研究了异搏定、红霉素、潘生丁、P gp单克隆抗体、复方丹参对MDR的逆转作用。结果 C6 /adrMDR细胞系mdr l基因阳性 ,P gp高度表达。异搏定 (2～ 6 μg/ml)、红霉素 (5 0～ 10 0 μg/ml)、潘生丁 5 μg/ml能够增加MDR细胞内阿霉素的浓度 ,且上述作用随浓度的增加而增强 ,其中红霉素 10 0 μg/ml作用最强 ,潘生丁 1μg/ml、P gp单克隆抗体、复方丹参无明显影响。结论 mdrl基因及其P gp蛋白的表达与胶质瘤细胞产生的MDR有关 ;异搏定、红霉素和潘生丁对胶质瘤细胞MDR有逆转作用
Objective To study the pathogenesis and reversal of multidrug resistance (MDR) in glioma. Methods The C6 / adrMDR cell line was established. The expression of mdr 1 gene and P glycoprotein (P gp) were studied by RT PCR and immunohistochemical staining respectively. The intracellular adriamycin concentration was determined by MTT drug sensitivity test and HPLCA The reversal effects of verapamil, erythromycin, dipyridamole, P gp monoclonal antibody and compound Danshen on MDR were studied. Results The mdr 1 gene of C6 / adrMDR cell line was positive and P gp was highly expressed. Verapamil (2 ~ 6 μg / ml), erythromycin (50 ~ 100 μg / ml) and dipyridamole 5 μg / ml increased the concentration of doxorubicin in MDR cells, and the above effects increased with increasing concentration Enhanced erythromycin 10 0 μg / ml strongest, dipyridamole 1 μg / ml, P gp monoclonal antibody, compound Salvia did not significantly affect. Conclusion The expression of mdrl gene and its P gp protein is related to the MDR produced by glioma cells. Verapamil, erythromycin and dipyridamole may reverse the MDR of glioma cells