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目的建立HPLC法同时测定金莲花中色氨酸、对羟基苯甲苹果酸、荭草素-2″-O-β-L-半乳糖苷、荭草素、牡荆素的含量。方法以Phenomenex Gemini C18色谱柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸水溶液梯度洗脱,流速1 ml·min-1,柱温35℃,检测波长230 nm。结果 5种成分均达到基线分离,线性关系良好(r≥0.998 7),平均加样回收率为95.05%~103.5%,RSD<3%。结论该方法灵敏、准确、可靠、重复性好,可用于金莲花药材的质量评价。
OBJECTIVE To establish a HPLC method for the simultaneous determination of tryptophan, p-hydroxybenzoic acid, andrographolide-2 "-O-β-L-galactoside, arbutin and vitexin.METHODS Phenomenex Gemini C18 column (4.6 mm × 250 mm, 5 μm) and gradient elution of acetonitrile-0.1% phosphoric acid in water with a flow rate of 1 ml · min-1 and a column temperature of 35 ° C. The detection wavelength was 230 nm. , The linearity was good (r≥0.998 7), the average recovery was 95.05% ~ 103.5%, RSD <3% .Conclusion The method is sensitive, accurate, reliable and reproducible.