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目的:优化灵芝发酵蕨渣基质生产菌质多糖的工艺条件。方法:以蕨渣为主要原料,采用响应面法优化生产菌质多糖的工艺条件(基质蕨渣比例、基质含水量和培养温度)。结果:基质蕨渣比例、基质含水量和培养温度对灵芝培养物中菌质多糖含量均有显著的影响(其中前者p<0.05,后两者p<0.01),且基质含水量与培养温度、基质蕨渣比例与基质含水量之间存在交互作用。优化的生产菌质多糖的工艺条件为蕨渣84.3%,基质含水量62.5%,培养温度28℃。在此条件下,灵芝培养物中菌质多糖的预测值为3.7117%,验证试验所得菌质多糖为3.6753%。回归方程的预测值和试验值差异不显著,所得回归模型拟合情况良好,达到设计要求。结论:首次利用蕨渣培养灵芝生产菌质多糖,该技术为利用蕨渣生产药用真菌菌质多糖提供技术支持。
OBJECTIVE: To optimize the technological conditions for the production of bacterial polysaccharides by fermenting fermented ferment slags with Ganoderma lucidum. Methods: Fermentation residue was used as the main raw material to optimize the process conditions (ratio of matrix fern residue, substrate water content and incubation temperature) by response surface methodology. Results: The ratio of substrate fern residue, substrate water content and culture temperature had significant effects on the content of polysaccharides in Ganoderma lucidum culture (p <0.05 for the former and p <0.01 for the latter two), and the relationship between the substrate water content and culture temperature, There is an interaction between the proportion of matrix fern dregs and the matrix water content. Optimized conditions for the production of bacterial polysaccharides fermented dregs of 84.3%, substrate moisture content of 62.5%, culture temperature 28 ℃. Under these conditions, the predicted value of bacterial polysaccharides in Ganoderma lucidum culture was 3.7117%, and that of the purified polysaccharides in the validation experiment was 3.6753%. There is no significant difference between the predicted value and the experimental value of the regression equation, and the obtained regression model fits well and meets the design requirements. CONCLUSION: The fermented dregs of Ganoderma lucidum were used for the first time to produce bacterial polysaccharides. This technology provides technical support for the production of polysaccharides from medicinal fungi using fern residue.