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为研究驻极体外静电场对猪胰岛素结构和介电特性的影响,从而为进一步研究驻极体提高胰岛素的降糖能力提供理论依据,以常温电晕充电下制备得到的500 V、1 000 V和1 500 V聚丙烯驻极体为电场源作用于胰岛素,测量药物的d33系数及核磁共振和非变性凝胶电泳谱。研究结果表明:1)不同表面电位聚丙烯驻极体产生的外静电场能使胰岛素溶液和胰岛素贴剂两侧形成相对稳定的静电场,电场强度与驻极体的等效表面电位成正比;2)驻极体外静电场对胰岛素的一级结构和二级结构没有明显影响;3)经500 V、1 000 V和1 500 V驻极体作用的胰岛素贴剂的d33值分别较胰岛素贴剂提高15.7倍、21.3倍和32.0倍;4)核磁共振谱表明驻极体作用后的胰岛素结构中氢键网络发生了改变。因此,驻极体产生的静电场在一定程度上引起了胰岛素分子的极化,改变了胰岛素的带电性,有利于胰岛素单体的形成,并能够提高胰岛素的降糖能力。
In order to study the effect of the electrostatic field outside the electret on the structure and dielectric properties of porcine insulin, this study provides a theoretical basis for further studies on the electret’s ability to enhance the hypoglycemic effect of insulin. The theoretical basis for the hypoglycemic effect of the electret at 500 V, 1000 V And 1 500 V polypropylene electret acting as an electric field source on insulin, measuring the d33 coefficient of the drug, and nuclear magnetic resonance and non-denaturing gel electrophoresis. The results show that: 1) The electrostatic field generated by polypropylene electrets with different surface potentials can form a relatively stable electrostatic field on both sides of the insulin solution and insulin patch, and the electric field strength is proportional to the equivalent surface potential of the electret. 2) the electrostatic field outside the electret has no obvious effect on the primary structure and the secondary structure of insulin; 3) the d33 values of the insulin patches treated with 500 V, 1 000 V and 1 500 V electrets are respectively higher than those of the insulin patch Increased by 15.7 times, 21.3 times and 32.0 times; 4) Nuclear magnetic resonance spectrum showed that the hydrogen bond network changed in the insulin structure after the electret action. Therefore, the electret electrostatic field generated to some extent caused by the polarization of insulin molecules, changing the charging of insulin, is conducive to the formation of insulin monomers, and can improve insulin hypoglycemic capacity.