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目的研究核黄素(Riboflavin)对高脂血症大鼠血脂水平的影响,为指导高脂血症人群膳食和预防心脑血管疾病提供科学依据。方法喂养高脂饲料建立高脂血症模型,将造模成功的大鼠随机分为核黄素剂量为0.677、3.385、6.770 mg/kg的干预组,同时设模型对照组、空白对照组,每组10只大鼠,干预60 d。核黄素干预组每日灌胃相应浓度的核黄素溶液,模型对照组和空白对照组灌胃等剂量的生理盐水。在不同时期,眼内眦采血(不禁食),测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平;实验末期眼内眦采血(禁食),测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平。结果与模型对照组[分别为TC:(3.41±0.56)、(3.39±0.58)mmol/L,LDL-C:(1.02±0.33)、(0.98±0.24)mmol/L]相比,0.677 mg/kg干预组第40、60天时血清TC[分别为(2.99±0.33)、(2.81±0.43)mmol/L]、LDL-C[分别为(0.82±0.10)、(0.79±0.11)mmol/L]水平明显降低,差异均有统计学意义(P<0.05)。干预末期,0.677 mg/kg干预组血清ALT水平[(35.70±5.31)U/L]明显低于模型对照组[(44.40±11.93)U/L],差异有统计学意义(P<0.05),各组间血清AST水平差异均无统计学意义(P>0.05)。结论适量的核黄素可降低高脂血症大鼠的血脂水平,预防心脑血管疾病的发生。
Objective To study the effect of riboflavin on the level of serum lipids in hyperlipidemic rats and to provide a scientific basis for the dietary and prevention of cardiovascular and cerebrovascular diseases in hyperlipidemic patients. Methods The model of hyperlipidemia was established by feeding high-fat diet. The successful rats were randomly divided into intervention group with riboflavin doses of 0.677, 3.355 and 6.770 mg / kg, and model control group and blank control group Group of 10 rats, intervention 60 d. In the riboflavin intervention group, the corresponding concentrations of riboflavin solution were given daily, and the model control group and the blank control group were given the same dose of normal saline. The levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were measured at different time points. (Fasting), serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in the end of the experiment. Results Compared with the model control group (TC: 3.41 ± 0.56, 3.39 ± 0.58 mmol / L, LDL-C: 1.02 ± 0.33 and 0.98 ± 0.24 mmol / L, (2.99 ± 0.33, 2.81 ± 0.43 mmol / L] and LDL-C [(0.82 ± 0.10) and (0.79 ± 0.11) mmol / L] The level was significantly lower, the difference was statistically significant (P <0.05). The level of serum ALT in the intervention group was significantly lower than that in the model control group ([44.40 ± 11.93] U / L] [(35.70 ± 5.31) U / L] There was no significant difference in serum AST levels between groups (P> 0.05). Conclusion An adequate amount of riboflavin can reduce the level of blood lipid in hyperlipidemic rats and prevent the occurrence of cardiovascular and cerebrovascular diseases.