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细胞周期素与相应的细胞周期素依赖性蛋白激酶相结合,驱动着细胞通过细胞周期各时相,而细胞周期素时相性规律大多来自酵母研究或同步化细胞的分析。本研究着重于人类非同步化细胞的细胞周期素时相性规律的揭示。采用人类白血病细胞株MOLT-4,使其处于对数生长期,加以有丝分裂中期阻滞法,应用多参数流式细胞术分析人类主要细胞周期素B1、A和E。分析发现,细胞周期素B1峰值在M期,降解于M期后,细胞周期素A峰值在G_2期,降解于M期,细胞周期素E峰值在G_1晚期,降解于S期。以上结果,使我们首次在人类非同步化培养细胞展现了主要细胞周期素的时相性表达规律。
Cyclins, in combination with the corresponding cyclin-dependent protein kinases, drive the passage of cells through the phases of the cell cycle, whereas the laws of the phase behavior of the cyclin are largely derived from yeast studies or from the analysis of synchronized cells. This study focuses on revealing the regularity of cyclins in human unsynchronized cells. The human leukemia cell line MOLT-4 was used in a logarithmic growth phase and mitotic metaphase arrest was applied to analyze human major cyclin B1, A and E using multi-parameter flow cytometry. The results showed that the peak of cyclin B1 was in phase M, the phase of degradation was in phase M, the peak of cyclin A was in phase G_2, degraded in phase M, the peak of cyclin E was in phase G_1 and degraded in S phase. The above results, for the first time in our human non-synchronized cultured cells show the expression of major cyclins in the phase of the phase.