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采用钙荧光指示剂Fura—2/Am定量测试法检测了静息淋巴细胞胞浆内游离钙浓度,并以Ca~(2+)载体A23187作为阳性对照.观察了(一)蝙蝠葛苏林碱(8701)对兔淋巴细胞胞浆内Ca~(2+)浓度的影响。结果表明:8701的各种实验浓度均可显著地促进淋巴细胞内Ca~(2+)浓度增加,在浓度为10~(-4)mol·L~(-1)时,增加细胞内Ca~(2+)浓度的作用显著高于Ca~(2+)载体A23187的作用(P<0.01),提示该药有可能作为淋巴细胞的刺激剂或在高浓度时可能造成淋巴细胞的Ca~(2+)中毒作用。
The calcium concentration was measured by the Fura-2 / Am assay, and the Ca 2+ -containing A23187 was used as a positive control.The effects of (1) batsurgentin (8701 ) On the cytosolic Ca ~ (2+) concentration in rabbit lymphocytes. The results showed that all the experimental concentrations of 8701 could significantly increase the intracellular Ca2 + concentration in lymphocytes, and increase the intracellular Ca ~ (2 +) concentration at the concentration of 10 ~ (-4) mol·L ~ (-1) (2+) concentration was significantly higher than that of Ca2 + carrier A23187 (P <0.01), suggesting that the drug may act as a stimulator of lymphocytes or at high concentrations may cause lymphocyte Ca ~ ( 2+) poisoning effect.