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[Objective] To compare the content of geniposide in FRUCTUS GARDENIAE before and after being processed.[Methods] The content of geniposide in FRUCTUS GARDENIAE was determined by HPLC.A HPLC analysis was carried out on a Inertsil ODS-2 C18 chromatographic column(250 mm × 4.6 mm,5 μm) with the detection wavelength at 430 nm and column temperature at 40 ℃.Following the injection of 20 μl of sample solution,an elution with acetonitrile-water(V/V,15:85) as mobile phase was initiated at a flow rate of 1.0 ml/min.[Result] Different processed products of FRUCTUS GARDENIAE varied in the content of curcumin and listed in a decreasing order as follows:raw materials(3.60%) > carbonized sample(3.36%)> parched sample(3.22%) > slightly brown-parched sample(3.02%) > yellow-parched sample(2.77%).[Conclusion] Different processed methods may have some effects on the content of geniposide in FRUCTUS GARDENIAE.
[Methods] The content of geniposide in FRUCTUS GARDENIAE was determined by HPLC. A HPLC analysis was carried out on a Inertsil ODS-2 C18 chromatographic column (250 mm × 4.6 mm, 5 μm) with the detection wavelength at 430 nm and column temperature at 40 ° C. After the injection of 20 μl of sample solution, an elution with acetonitrile-water (V / V, 15:85) as mobile phase was initiated at a flow rate of 1.0 ml / min. [Result] Different processed products of FRUCTUS GARDENIAE varied in the content of curcumin and listed in a decreasing order as follows: raw materials (3.60%)> carbonized sample (3.36%)> parched sample (3.22%)> slightly brown-parched sample (3.02%)> yellow-parched sample (2.77%). [Conclusion] Different processed methods may have some effects on the content of geniposide in FRUCTUS GARDENIAE.