我们自1976年在工作中发现,每管接种花药数多的比每管接种花药数少的愈伤组织诱导率高,表明花药聚集有提高诱导率的效果。为了证明这种效果,我们进一步作了有关方面的试验。试验材料取自本所水稻常规育种组的(红飞娥糯×红410)F_1和(红飞娥糯×珍珠糯)F_1,这是两个糯×籼、糯×糯组合,在花粉处于单核晚期进行接种。基本培养基选用 N_6、籼诱Ⅲ(广东植物所)。以每管花药40—50枚的为对照,聚集效应处理的每管花药80—85枚。花药培养按一般方法进行,花药接 We have found in our work since 1976 that the induction rate of many calluses inoculated per tube is less than the number of inoculated anthers in each tube, indicating that anther aggregation has the effect of increasing the induction rate. In order to prove this effect, we have further made relevant experiments. The test materials were obtained from the conventional breeding group (Hongfei E Nuo × Red 410) F_1 and (Hongfei E Nuo × Pear Nuo) F_1, which are two waxy × indica, waxy × waxy combinations. When the pollen is in a single Late stage of nuclear vaccination. The basic medium selection N_6, Indica induction Ⅲ (Guangdong Institute of Botany). 40-50 anthers per tube were used as controls, and 80-85 anthers were collected for each effect. Anther culture according to the general method, anther then