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目的评价噻唑橙(TO)光化学法病原体灭活处理对红细胞质量的影响。方法设实验组(n=10):悬浮红细胞经TO光化学法处理后,在4℃储存0、7、14、21、28、35 d时,分别测定pH、溶血率、钾浓度、ATP浓度、2,3-DPG浓度;对照组(n=10):悬浮红细胞未经TO光化学法处理,测定时间、项目同实验组。结果保存过程中实验组pH、2,3-DPG(μmol/gHb)及ATP(μmol/gHb)下降趋势均与对照组差异不大(P>0.05),实验组分别从0 d的7.03±0.07、9.43±1.14、4.23±0.58下降到35 d的6.57±0.06、0、2.40±0.49,对照组分别从0 d的6.95±0.09、10.67±1.17、5.18±0.64,下降到35 d的6.57±0.07、0、2.99±0.44;实验组和对照组35 d储存期末ATP浓度分别为0 d时的56.7%和57.7%。;实验组在储存期末,溶血率为(0.27±0.06)%,略高于对照组0.11±0.04%(P<0.05),仍满足溶血率≤0.8%的国标要求;钾外漏实验组较对照组增加,35 d时分别为(40.41±2.94)mmol/L和(28.33±3.34)mmol/L(P<0.01)。结论 TO光化学法处理对储存过程中的红细胞的质量影响较小。
Objective To evaluate the effect of thiazole orange (TO) photochemical pathogen inactivation on erythrocyte quality. Methods The experimental group (n = 10) was established. After the erythrocytes were treated with TO photochemical method and stored at 4 ℃ for 0, 7, 14, 21, 28 and 35 d, the pH, the hemolysis rate, the potassium concentration, the ATP concentration, The concentration of 2,3-DPG in the control group (n = 10): The suspension of erythrocytes without TO photochemical treatment, the determination of time, the project with the experimental group. Results The decreasing tendency of pH, 2,3-DPG (μmol / gHb) and ATP (μmol / gHb) in the experimental group were not significantly different from those in the control group (P> 0.05) , 9.43 ± 1.14, 4.23 ± 0.58, respectively, decreased to 6.57 ± 0.06,0,2.40 ± 0.49 on the 35th day, and decreased from 6.95 ± 0.09,10.67 ± 1.17,5.18 ± 0.64 on the 0th day to 6.57 ± 0.07 , 0, 2.99 ± 0.44; The ATP concentration of the experimental group and the control group at the end of storage period were 35.6% and 57.7% respectively at 0 day. (0.27 ± 0.06)%, slightly higher than that of the control group (0.11 ± 0.04%) (P <0.05), still meet the national standard of hemolysis rate less than 0.8%; the experimental group of potassium leakage was better than the control Group was (40.41 ± 2.94) mmol / L and (28.33 ± 3.34) mmol / L respectively on the 35th day (P <0.01). Conclusion TO photochemical treatment has little effect on the quality of red blood cells during storage.