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目的:考察鞣花酸对糖尿病小鼠肾脏的保护作用及其机制。方法:40只雄性ICR小鼠随机分为正常对照组、模型对照组、厄贝沙坦组(20 mg·kg~(-1))和鞣花酸组(100 mg·kg~(-1)),每组10只。其中模型对照组、厄贝沙坦组和鞣花酸组采用单次腹腔注射链脲霉素(180 mg·kg~(-1))的方法建立糖尿病小鼠模型。注射3周后厄贝沙坦组和鞣花酸组进行给药干预,正常对照组和模型对照组给予生理盐水,灌胃给药,1次/d,连续4周。观察小鼠一般状态、血糖及体质量,肾组织行HE和PAS染色观察肾脏病理形态,测定尿微量白蛋白、血清和尿样中尿素氮、肌酐、血清和肾脏组织中MDA和T-SOD,并计算肾脏指数。结果:模型对照组较正常对照组血糖、24 h尿微量白蛋白、肾脏指数、血肌酐、血尿素氮、MDA明显升高(P<0.01),体质量、尿肌酐、尿素氮、T-SOD明显降低(P<0.01)。与模型对照组相比,鞣花酸组和厄贝沙坦组小鼠的状态明显好转,系膜增生明显缓解,24 h微量白蛋白明显下降(P<0.01),血肌酐明显下降(P<0.05或P<0.01),尿肌酐和T-SOD明显升高(P<0.05或P<0.01),MDA明显降低(P<0.01)。结论:鞣花酸对糖尿病小鼠的肾损伤具有一定的缓解作用,可能与抗氧化机制有关。
Objective: To investigate the protective effect of ellagic acid on the kidney of diabetic mice and its mechanism. Methods: Forty male ICR mice were randomly divided into normal control group, model control group, irbesartan group (20 mg · kg -1) and ellagic acid group (100 mg · kg -1) ), 10 in each group. The diabetic mice model was established by intraperitoneal injection of streptozotocin (180 mg · kg -1) in model control group, irbesartan group and ellagic acid group. Irbesartan group and ellagic acid group were administrated for 3 weeks after injection. Normal control group and model control group were given physiological saline, gavage once a day for 4 weeks. The general state of mice, blood glucose and body weight were observed. The renal tissues were stained with HE and PAS to observe the renal pathological changes. The levels of MDA and T-SOD in urine microalbumin, serum and urine samples were measured, And calculate the kidney index. Results: Compared with normal control group, blood glucose, 24h urinary albumin, renal index, serum creatinine, blood urea nitrogen and MDA were significantly increased (P <0.01), body mass, creatinine, urea nitrogen, T-SOD Significantly lower (P <0.01). Compared with the model control group, the mice in ellagic acid group and irbesartan group improved significantly, the mesangial hyperplasia relieved significantly, the 24 h microalbumin decreased significantly (P <0.01), and the serum creatinine decreased significantly (P < 0.05 or P <0.01). Urinary creatinine and T-SOD were significantly increased (P <0.05 or P <0.01) and MDA significantly decreased (P <0.01). Conclusion: Ellagic acid can relieve the renal injury of diabetic mice and may be related to the anti-oxidative mechanism.