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目的:建立桑叶中芦丁含量的测定方法。方法:高效液相色谱法,以VARIAN ODS(250 mm×4.6 mm,5μm)为色谱柱,乙腈(A)-0.5%磷酸(B)做流动相,经梯度洗脱[0.01 min,A-B(18∶82);4 min,A-B(21∶79);14 min,A-B(8∶92);17 min,A-B(18∶82);18 min,A-B(18∶82)],流速1.0 ml/min,检测波长为358 nm,柱温为40℃,紫外检测器检测。结果:芦丁浓度5μg/ml～100μg/ml范围内呈良好的线性关系,(r=0.99996,n=5),精确度(RSD=1.9%～2.1%),平均回收率试验为102%。最低检出限为0.1μg/ml(以进量浓度计)。结论:实验结果表明该方法灵敏度高,精密度和准确度好,是一种简便、快速分析的方法。
Objective: To establish a method for the determination of rutin in mulberry leaves. Methods: The mobile phase consisted of VARIAN ODS (250 mm × 4.6 mm, 5 μm) column and acetonitrile (A) - 0.5% phosphoric acid (B) (18:82); AB (18:82); AB (18:82); flow rate 1.0 ml / min , Detection wavelength of 358 nm, column temperature of 40 ℃, UV detector detection. Results: A good linear relationship was found between rutin concentration 5μg / ml and 100μg / ml (r = 0.99996, n = 5) and accuracy (RSD = 1.9% ~ 2.1%). The average recovery was 102%. The minimum detection limit of 0.1μg / ml (in volume concentration). Conclusion: The experimental results show that the method is sensitive, accurate and accurate, which is a simple and rapid method.