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研究中药左金丸活性成分巴马汀和小檗碱单独与联合对人胃癌细胞MGC803生长的抑制作用。方法:体外培养人胃癌细胞MGC803,通过MTT比色法实验检测巴马汀、小檗碱以及两药联合对人胃癌细胞MGC803的增殖抑制作用,分别计算出半抑制浓度IC50,利用Chou和Talalay法计算两种药物的合用指数(CI)。结果:巴马汀、小檗碱以及两药联合对MGC803的增殖均有抑制作用,并呈剂量依赖关系。其IC50分别为69.02μ mol/L、54.08μ mol/L和37.67μ mol/L。当两药低浓度(<14.79μ mol/L)联合时CI>1,表明巴马汀和小檗碱有拮抗作用;当两药高浓度(>14.79μ mol/L)联合时CI<1,表明巴马汀和小檗碱有协同作用。结论:巴马汀、小檗碱及两药联合对人胃癌细胞MGC803的增殖有抑制作用。
To study the inhibitory effect of palmatine and berberine alone or in combination on the growth of human gastric cancer cell line MGC803. Methods: Human gastric cancer cell line MGC803 was cultured in vitro. The inhibitory effects of palmatine and berberine on the proliferation of human gastric cancer cell line MGC803 were assayed by MTT colorimetric assay. The half-inhibitory concentration (IC50) was calculated. Chou and Talalay method Calculate the combined index (CI) of the two drugs. Results: Palmatine, berberine and their combination inhibited the proliferation of MGC803 in a dose-dependent manner. The IC50 were 69.02μ mol / L, 54.08μ mol / L and 37.67μ mol / L, respectively. When CI> 1 at low concentrations (<14.79 μ mol / L), the combination of palmatine and berberine showed antagonism; CI <1 when combined with high concentrations (> 14.79 μ mol / L) Showed that palmatine and berberine have synergistic effects. Conclusion: Palmatine, berberine and their combination inhibit the proliferation of human gastric cancer cell line MGC803.