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目的观察不同质量浓度的氯化镉(CdCl2)染毒后人胚肾细胞(HEK 293T细胞)经典瞬时受体电位通道(TRPC)6个亚型mRNA表达水平的改变,在mRNA水平上探讨镉对肾细胞钙通道的影响。方法 HEK 293T细胞分别暴露于7.5、15.0、30.0、45.0和60.0μmol/L的CdCl28 h后,应用四甲基偶氮唑蓝(MTT)比色法测定细胞增殖活力;实时荧光定量聚合酶链反应法检测0、15.0和30.0μmol/L CdCl2作用8 h和30.0μmol/L CdCl2染毒4 h后TRPC 6个亚型mRNA表达水平。结果以对照组(CdCl20μmol/L)HEK 293T细胞存活率为100%,各不同质量浓度的染毒组细胞存活率分别为79.40%、77.29%、72.26%、70.25%、60.80%,与对照组比较,差异有统计学意义(P<0.05或P<0.01)。经CdCl2染毒后,各染毒组HEK 293T细胞的TRPC 6个亚型mRNA相对表达量均低于对照组(P<0.05或P<0.01)。结论 CdCl2可能引起HEK 293T细胞的TRPC基因mRNA的表达水平发生改变。
OBJECTIVE: To investigate the changes of mRNA expression of 6 subtypes of classic transient receptor potential channel (TRPC) in human embryonic kidney (HEK 293T) cells exposed to different concentrations of cadmium chloride (CdCl2) Effects of calcium channels in renal cells. METHODS HEK 293T cells were exposed to 7.5, 15.0, 30.0, 45.0 and 60.0 μmol / L of CdCl for 28 h, respectively. The proliferation of HEK 293T cells was detected by MTT assay. Real-time fluorescence quantitative PCR The mRNA expression levels of TRPC six subtypes after treated with 0, 15.0 and 30.0 μmol / L CdCl2 for 8 h and 30.0 μmol / L CdCl2 for 4 h were assayed. Results The survival rate of HEK 293T cells in control group (CdCl20μmol / L) was 100%. The cell viabilities in different concentration groups were 79.40%, 77.29%, 72.26%, 70.25% and 60.80%, respectively. Compared with the control group , The difference was statistically significant (P <0.05 or P <0.01). After exposure to CdCl2, the mRNA expression of TRPC6 subtypes in HEK 293T cells was lower than that in the control group (P <0.05 or P <0.01). Conclusion CdCl2 may cause the expression of TRPC mRNA in HEK 293T cells to change.