论文部分内容阅读
利用实时荧光定量PCR技术,结合geNorm、NormFinder和BestKeeper软件研究了5个常用的植物内参基因GAPDH、β-Actin、18S rRNA、UBQ和EF-1在不同基因型、不同组织、不同发育时期核桃(Juglans spp.)中的表达情况,以期筛选出在核桃中稳定表达的内参基因。结果显示:GAPDH和EF-1在核桃所有样品中均表达稳定,由于表达丰度偏低,所以适合作为分析较低表达丰度目标基因时的内参;18S rRNA在核桃所有样品中表达稳定性较好,且18S rRNA表达丰度偏高,适合作为分析较高表达丰度目标基因时的内参;β-Actin和UBQ在核桃样品中的表达稳定性较差,不适合作为核桃中基因表达分析时的内参基因。
Five commonly used plant reference genes, GAPDH, β-Actin, 18S rRNA, UBQ and EF-1, were detected in real-time fluorescence quantitative PCR and geNorm, NormFinder and BestKeeper softwares in different genotypes, different tissues and different developmental stages Juglans spp.), In order to screen out the stable expression of the reference gene in walnut. The results showed that GAPDH and EF-1 were stable in all walnut samples, and were suitable as internal control for analyzing the lower expression abundance genes because of low expression abundance. The stability of 18S rRNA expression in all samples of walnut , And 18S rRNA gene expression abundance is high, suitable as a reference gene for analysis of higher expression abundance; β-Actin and UBQ in walnut samples expression stability is poor, not suitable as a gene expression analysis in walnut Of the internal reference gene.