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目的探讨江苏省汉族人群PPARγ基因外显子区两个单核甘酸多态性(rs1801282,rs3856806)与2型糖尿病(T2DM)的关系。方法采用病例对照研究选取新发T2DM 296例,年龄、性别频数匹配的健康对照477名,用PCR-RFLP方法进行两位点多态性检测。结果 PPARγ基因rs3856806位点多态性在病例组与对照组的分布差异有统计学意义(P<0.05)。与携带CC型相比较,携带CT/TT型者患T2DM风险降低。分层分析发现在女性、>50岁、高血压或体重正常群体中,携带CT/TT型者较CC型者患T2DM风险降低[OR值分别为0.33(95%CI:0.14,0.76),0.45(95%CI:0.23,0.88)、0.59(95%CI:0.36,0.95),0.34(95%CI:0.13,0.94)]。rs1801282位点多态性在病例组和对照组中的分布差异无统计学意义(P>0.05),但分层分析发现,携带CG(Pro/Ala)型肥胖者较CC型者患T2DM发病风险降低[OR(95%CI)=0.30(0.09,0.90)]。结论 PPARγ基因多态性改变可能与江苏汉族人群T2DM遗传易感性有关。
Objective To investigate the relationship between two SNPs (rs1801282, rs3856806) and type 2 diabetes mellitus (T2DM) in exon of PPARγ gene of Han nationality in Jiangsu Province. Methods A case-control study was conducted to select 296 newly diagnosed cases of T2DM and 477 healthy controls matched for age and sex frequency. Two-locus polymorphism was detected by PCR-RFLP. Results The rs3856806 polymorphism of PPARγ gene was significantly different between the case group and the control group (P <0.05). Patients with CT / TT type have a reduced risk of T2DM compared with those with CC type. Stratified analysis showed a lower risk of T2DM among women with> 50 years of age, high blood pressure, or normal weight with CT / TT type compared with CC type [OR = 0.33 (95% CI: 0.14, 0.76) (95% CI: 0.23, 0.88), 0.59 (95% CI: 0.36, 0.95), 0.34 (95% CI: 0.13, 0.94)]. There was no significant difference in the distribution of rs1801282 between the case group and the control group (P> 0.05). However, stratified analysis showed that the risk of T2DM in patients with CG (Pro / Ala) Reduce [OR (95% CI) = 0.30 (0.09, 0.90)]. Conclusion The changes of PPARγ gene polymorphism may be related to the genetic susceptibility to T2DM in Han population in Jiangsu Province.