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目的 研究人类白细胞抗原DQA1(HLA DQA1)等位基因与桥本甲状腺炎 (HT)的相关性。方法 采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)基因分型技术 ,对 5 1例HT患儿及5 1名正常儿童对照进行了HLA DQA1基因分型并计算比较其基因频率。所有研究对象均为天津汉族儿童。结果 在HT组 ,HLA DQA1 0 30 1等位基因频率明显高于正常对照 (44 12 %对 2 8 43% ,RR =1 99,P <0 0 2 5 )而HLA DQA1 0 2 0 1等位基因频率则明显低于正常对照 (5 88%对 18 6 3% ,RR =0 2 7,P <0 0 1)。结论 HLA DQA1 0 30 1可能是HT的一个易感等位基因 ,而HLA DQA1 0 2 0 1则可能是一个保护等位基因 ,HT的发病与HLA DQA1基因位点密切相关。
Objective To investigate the association between human leukocyte antigen DQA1 (HLA DQA1) alleles and Hashimoto’s thyroiditis (HT). Methods PCR RFLP genotyping was used to genotype HLA DQA1 in 51 children with HT and 51 normal children. The gene frequency was calculated and compared. All subjects were Han Chinese children in Tianjin. Results In HT group, the frequency of HLA DQA1 0 30 1 allele was significantly higher than that of control (44 12% vs 28 43%, RR = 1 99, P 0 052) and HLA DQA 1 0 0 0 1 1 The gene frequency was significantly lower than the normal control (58.8% vs. 18.63%, RR = 0.27, P <0.01). Conclusion HLA DQA1 0 30 1 may be a susceptible allele of HT, while HLA DQA1 0 0 01 may be a protective allele. The incidence of HT is closely related to the HLA DQA1 gene locus.