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目的:研究香加皮杠柳苷(CPP)对荷瘤小鼠的免疫调节作用及其作用机制。方法:建立BALB/c小鼠H22皮下移植瘤,观察低、中、高剂量CPP(0.25、0.50、1.00mg/kg)对荷瘤小鼠免疫器官的影响,应用流式细胞术检测各组小鼠脾T淋巴细胞亚群的分布,应用MTT法检测ConA诱导的小鼠脾淋巴细胞增殖活性,用ELISA试剂盒测定各组荷瘤小鼠血清中细胞因子TNF-α、IL-2和IL-12的含量。结果:对照组荷瘤小鼠的胸腺指数及脾脏指数均明显低于未荷瘤正常小鼠(P<0.05),而CPP组荷瘤小鼠的胸腺指数及脾脏指数较对照组明显增高,甚至高于正常对照组(P<0.05)。CPP对荷瘤小鼠体内CD8+T细胞数量无影响,但可明显上调CD3+、CD4+T细胞数及CD4+/CD8+比值,其中CD3+、CD4+细胞百分率与正常小鼠无差别(P>0.05),CD4+/CD8+比值高于正常小鼠(P<0.05)。CPP可明显增强ConA诱导的荷瘤小鼠脾淋巴细胞的增殖能力,SI甚至超过正常对照组(P<0.05)。不同剂量CPP组小鼠血清中TNF-α、IL-2和IL-12水平均较对照组明显增高(P<0.05),并随剂量增加呈升高趋势,至接近或超过正常小鼠水平。结论:CPP可保护荷瘤小鼠的免疫器官不受损害,并可明显提高CD4+T细胞百分率和CD4+/CD8+比值,增强荷瘤小鼠T细胞增殖能力,促进细胞因子TNF-α、IL-2和IL-12的产生,表明CPP具有显著的免疫增强作用。
Objective: To study the immunoregulatory effects and its mechanism of Periplocin (CPP) on tumor-bearing mice. METHODS: BALB/c mouse H22 subcutaneously transplanted tumors were established. The effects of low, middle, and high doses of CPP (0.25, 0.50, and 1.00 mg/kg) on the immune organs of tumor-bearing mice were examined. Flow cytometry was used to detect the effects of each group. Distribution of mouse spleen T lymphocyte subsets. MTT assay was used to detect the activity of ConA-induced mouse spleen lymphocyte proliferation. The TNF-α, IL-2 and IL-2 levels in the serum of tumor-bearing mice in each group were determined by ELISA kit. 12 levels. RESULTS: The thymus index and spleen index of the tumor-bearing mice in the control group were significantly lower than those of the normal mice without tumor (P<0.05), while the thymus index and spleen index of the tumor-bearing mice in the CPP group were significantly higher than those in the control group. Higher than the normal control group (P<0.05). CPP had no effect on the number of CD8+ T cells in tumor-bearing mice, but significantly increased the number of CD3+ and CD4+ T cells and the ratio of CD4+/CD8+. The percentage of CD3+ and CD4+ cells had no difference with normal mice (P>0.05). The ratio of CD4+/CD8+ was higher than that of normal mice (P<0.05). CPP significantly enhanced the proliferation of splenic lymphocytes in tumor-bearing mice induced by ConA. SI even surpassed the normal control group (P<0.05). The serum levels of TNF-α, IL-2 and IL-12 in mice of different doses of CPP group were significantly higher than those in the control group (P<0.05), and increased with the increase of dose, reaching or exceeding the level of normal mice. Conclusion: CPP can protect the immune organs of tumor-bearing mice from damage, and can significantly increase the percentage of CD4+ T cells and CD4+/CD8+ ratio, enhance the proliferation of T cells in tumor-bearing mice, and promote the cytokines TNF-α, IL- The production of 2 and IL-12 indicates that CPP has a significant immunoenhancing effect.