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目的采用HPLC法测定清热解毒口服液中黄芩苷、绿原酸、连翘苷、连翘酯苷A的含量。方法采用Zorbax Eclipse XDB C18色谱柱(150 mm×4.6 mm,5μm),柱温30℃,流动相为乙腈-0.3%磷酸溶液,梯度洗脱,流速1.0 m L·min~(-1),检测波长324 nm(绿原酸、连翘酯苷A)、276 nm(黄芩苷、连翘苷)。结果黄芩苷、绿原酸、连翘苷、连翘酯苷A的线性范围分别为0.182~0.912、0.061~0.304、0.037~0.183、0.013~0.065μg,加样回收率分别为98.82%、96.94%、105.6%、95.74%(n=6)。结论所用方法操作简单,为全面控制清热解毒口服液的质量提供了一种可靠的检测方法。
Objective To determine the contents of baicalin, chlorogenic acid, forsythin, forsythoside A in Qingrejiedu Oral Liquid by HPLC. Methods A Zorbax Eclipse XDB C18 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.3% phosphoric acid with gradient elution at a flow rate of 1.0 mL · L -1 at 30 ℃. Wavelength 324 nm (chlorogenic acid, forsythoside A), 276 nm (baicalin, forsythin). Results The linear ranges of baicalin, chlorogenic acid, forsythin and forsythiaside A were 0.182 ~ 0.912,0.061 ~ 0.304,0.037 ~ 0.183,0.013 ~ 0.065μg, respectively. The recoveries were 98.82%, 96.94% , 105.6%, 95.74% (n = 6). Conclusion The method is easy to operate and provides a reliable method for the comprehensive control of the quality of Qingrejiedu Oral Liquid.