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应用RT PCR扩增 3例异基因造血干细胞移植术后合并慢性GVHD患者的外周血单个核细胞的TCRVβ 2 4个亚家族的CDR3,了解患者TCRVβT细胞的表达 ,PCR产物进一步经基因扫描分析确定T细胞克隆性。结果显示 ,3例患者均有克隆性T细胞生长 ,它可能与GVHD的发生有关。
RT-PCR was used to amplify CDR3 of TCRVβ2 4 subfamilies of peripheral blood mononuclear cells in 3 patients with chronic GVHD after allogeneic hematopoietic stem cell transplantation to understand the expression of TCRVβT cells. PCR products were further analyzed by gene scanning to determine the T Cell clonality. The results showed that all three patients had clonal T cell growth, which may be related to the occurrence of GVHD.