采用人胚大脑神经细胞体外无血清原代分离培养方法，研究了不同剂量牛磺酸及不同培养天数对人大脑神经细胞衰老的影响。结果发现：培养神经细胞到第６，９，１５天时，牛磺酸组大脑神经细胞超氧化物歧化酶（ＳＯＤ）的活性均显著高于对照组（Ｐ＜０．０５）；而加入了不同浓度牛磺酸各组的神经细胞到培养的第６天时，与对照组相比，加入牛磺酸浓度为５０ｍｇ／Ｌ及２００ｍｇ／Ｌ的神经细胞组ＳＯＤ活性均显著高于对照组（Ｐ＜０．０５）。在培养到第三天时，牛磺酸组脑神经细胞中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性显著高于对照组（Ｐ＜０．０５），培养到第６和第９天时也呈现比对照组增高的趋势。本研究发现随着培养时间的延长，对照组神经细胞膜脂流动性显著下降，而牛磺酸组基本保持稳定；含不同牛磺酸浓度各组的神经细胞，在培养到第６天时，以含牛磺酸５０、２００及４００ｍｇ／Ｌ的神经细胞膜脂流动性较稳定，与对照组相比差异显著（Ｐ＜０．０１或Ｐ＜０．０５）。本研究首次证明，适宜剂量牛磺酸对维持神经细胞膜脂的正常流动性是必需的，可显著延缓大脑神经细胞的衰老。 The primary culture of human embryonic brain neurons in vitro without serum was used to study the effects of different doses of taurine and different culture days on the neuronal senescence of human brain. The results showed that the activity of superoxide dismutase (SOD) in neurons of taurine group was significantly higher than that of the control group (P <0.05) on the 6th, 9th and 15th day. Compared with the control group, the activity of SOD in nerve cell of taurine concentration group was significantly higher than that of the control group on the 6th day when cultured in the taurine concentration of 50mg / L and 200mg / L (P < 0.05). During the third day of culture, glutathione peroxidase (GSH-Px) activity in brain cells of taurine group was significantly higher than that of control group (P <0.05). On the sixth and ninth days Showing an upward trend than the control group. The study found that with the extension of culture time, the control group neuronal cell membrane fluidity decreased significantly, while the taurine group remained stable; containing different concentrations of taurine in each group of nerve cells, cultured to the first 6 days, containing The fluidity of membrane lipids in 50, 200 and 400 mg / L taurine was stable compared with the control group (P <0.01 or P <0.05). This study demonstrated for the first time that proper doses of taurine are necessary to maintain the normal fluidity of membrane lipids in nerve cells and can significantly delay the senescence of neuronal cells in the brain.