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目的基于叔胺结构的5-HT3拮抗剂对三联吡啶钌溶液的电化学发光信号具有显著增敏作用,建立应用毛细管电泳-电化学发光方法同时检测分析2种5-HT3拮抗剂盐酸格拉司琼、盐酸阿扎司琼的方法。方法采用正交实验设计,对毛细管电泳分离条件和电化学发光检测条件进行了优化。结果在优化的实验条件下,可在200s内实现盐酸格拉司琼和盐酸阿扎司琼的分离和检测,其质量浓度线性范围为3.0×10-7～1.0×10-4 g.mL-1;线性方程分别为I=48.990C+47.365(r=0.995 9)和I=52.605C+105.70(r=0.991 8);检出限分别为5.6(S/N=3)和3.9ng.mL-1(S/N=3);平均回收率分别为98.9%和101.3%,其RSD分别为0.74%和1.29%。结论该法灵敏简便,具有较强专属性,可同时检测盐酸格拉司琼和盐酸阿扎司琼。
OBJECTIVE: To investigate the effect of 5-HT3 antagonist on the electrochemiluminescence (PL) luminescence of terbium (R) bipyridyl ruthenium (TRI), and to establish a method for simultaneous determination of two 5-HT3 antagonists, granisetron hydrochloride , Azathiazole hydrochloride method. Methods The orthogonal experimental design was used to optimize the conditions of capillary electrophoresis separation and electrochemical detection. Results Under the optimized experimental conditions, granisetron hydrochloride and azasetron hydrochloride were separated and detected within 200s with a linear range of 3.0 × 10-7-1.0 × 10-4 g · mL-1 ; The linear equations were I = 48.990C + 47.365 (r = 0.995 9) and I = 52.605C + 105.70 (r = 0.991 8) .The detection limits were 5.6 (S / N = 3) and 3.9 ng.mL- 1 (S / N = 3). The average recoveries were 98.9% and 101.3%, respectively, with RSDs of 0.74% and 1.29%, respectively. Conclusion The method is sensitive and simple, with strong specificity, which can detect granisetron hydrochloride and atosine hydrochloride.