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目的:观察急性胆管炎时大鼠厅组织ICAM司与循环PMN表面CD11b、CD18的表达变化。方法:应用原位分子杂交和斑点杂交技术观察胆道感染时ICAM-1mRNA在肝组织的定位及转录表达强度;用流式细胞仪测定PMN表达CD11b及CD18的阳性年。结果:ICAM-1mRNA原位杂交显示胆道感染3h后肝表内皮细胞、枯否细胞和肝小叶中央静脉内皮细胞阳性反应增强，12h阳性反应最强.斑点杂交显示胆道感染后3h肝组织ICAM-1mRNA含量开始增高，12h达高峰.循环PMN表达CD11b和CD18的阳性率3h以后明显增高。结论:胆道感染大鼠肝表内皮细胞等表面ICAM-1与循环PMN表面CD11b、CD18的表达显著增强。
OBJECTIVE: To observe the expression of CD11b and CD18 on the surface of ICAM and circulating PMN in acute cholangitis rats. Methods: In situ hybridization and dot blot hybridization were used to observe the localization of ICAM-1mRNA in liver tissues and the intensity of transcriptional expression. The positive cells of CD11b and CD18 were detected by flow cytometry. Results: ICAM-1 mRNA in situ hybridization showed that the positive reaction of endothelial cells, Kupffer cells and central venous endothelial cells of hepatic lobule increased after 3 hours of biliary tract infection, and the positive reaction was most pronounced at 12 hours after 3 hours of biliary tract infection.It showed that ICAM-1 mRNA The content began to increase and peaked at 12h.The positive rate of CD11b and CD18 expression in circulating PMN was significantly increased after 3h. Conclusion: The expressions of ICAM-1 and CD11b and CD18 on the surface of liver-derived endothelial cells in the biliary tract infected rats were significantly increased.