There is a growing need to eliminate antibiotic resistance genes (ARGs) in the environment and mitigate widespread antibiotic resistance. Graphitic carbon nitride (g-C3N4) was successfully synthesized via facile thermal polymerization approach and its potential for adsorption treatment of ARGs in water was examined. Batch adsorption experimental results revealed that g-C3N4 powders had robust adsorption activity for the gene ampC and ermB. Adsorption kinetics and isotherms were systematically investigated to explain the adsorption mechanism. The apparent adsorption equilibrium could be reached within 180 min. The adsorption process effectively removed ARGs (ampC and ermB) from water with 3.2 log and 4.2 log reductions, respectively. In addition, experimental data were analyzed by several models and simulated well with Langmuir isotherm and pseudo-second-order model. It indicated that adsorption process might be dominated by the chemical rate-limiting step. Moreover, the effects of temperature and pH on the removal of ARGs were conducted and the isoelectric point (IEP) was obtained. Finally, we have demonstrated that the g-C3N4 is a novel adsorbent and can be used as column packing to remove ARGs by filtration.