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目的 探讨亚低温对肝缺血再灌注损伤的保护作用机制。方法 将 18只犬随机分为 3组 :非缺血对照组 (n =6 )、缺血再灌注组 (n =6 )和亚低温处理组 (肝周充填碎冰块造成肝脏亚低温 ,n =6 )。对各组肝上下腔静脉血进行谷丙转氨酶 (ALT)、谷草转氨酶(AST)、乳酸脱氢酶 (LHD )以及丙二醛 (MDA)和超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化酶 (GSH PX)活性及总抗氧化 (TAX )能力测定。结果 全肝缺血再灌注后ALT ,AST ,LDH和MDA含量明显上升 (P <0 .0 1) ,SOD ,CAT ,GSH PX活性及TAX能力明显下降 (P <0 .0 1) ;而亚低温处理组与缺血再灌注组比较 ,ALT ,AST ,LDH和MDA含量明显下降 (P <0 .0 1) ,SOD ,CAT ,GSH PX活性及TAX能力明显上升 (P <0 .0 1,P <0 .0 5 )。结论 亚低温能增强肝组织自身抗氧化能力 ,减轻肝缺血再灌注后氧自由基对肝脏的损伤。
Objective To investigate the protective effect of mild hypothermia on liver ischemia-reperfusion injury. Methods Eighteen dogs were randomly divided into three groups: non-ischemic control group (n = 6), ischemia reperfusion group (n = 6) and mild hypothermia group = 6). The levels of ALT, AST, LHD, MDA and SOD, catalase (CAT), catalase (CAT), glutathione peroxidase (GSH PX) activity and total antioxidant capacity (TAX) were measured. Results The levels of ALT, AST, LDH and MDA were significantly increased (P <0.01), and the activities of PX, TAX and activities of SOD, CAT, GSH were significantly decreased after the whole liver ischemia / reperfusion (P <0.01) The levels of ALT, AST, LDH and MDA in the hypothermia group were significantly decreased compared with those in the ischemia / reperfusion group (P <0.01), while the activity of PX, TAX and the activities of SOD, CAT, GSH were significantly increased (P < P <0. 05). Conclusion Mild hypothermia can enhance the anti-oxidative capacity of liver tissue and alleviate the liver damage caused by oxygen free radicals after hepatic ischemia-reperfusion.