用毛细管气相色谱法对十四碳到二十二碳的脂肪酸甲酯进行了分析，用气质联用法考察了用等效链长度（ＥＣＬ）值对未知脂肪酸进行定性的可能性，及银化薄层色谱法（ＡｇＴＬＣ）分离脂肪酸的特点。结果表明，用等效链长度值可以对大多数未知脂肪酸进行定性。并提出结合银化薄层色谱法和用毛细管气相色谱法对生物样品中复杂脂肪酸成分进行分析的方法 The fourteen to twenty carbon fatty acid methyl esters were analyzed by capillary gas chromatography. The possibility of qualitatively determining the unknown fatty acids using equivalent chain length (ECL) values ​​was investigated by GC-MS, Separation of fatty acids by layer chromatography (AgTLC). The results show that most of the unknown fatty acids can be characterized by the equivalent chain length. And proposed combining silver thin layer chromatography and capillary gas chromatography in biological samples of complex fatty acid composition analysis