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观察蛋氨酸脑啡肽(MENK)对巨噬细胞-粒细胞集落刺激因子(GM-CSF)诱导的小鼠骨髓来源树突状细胞(DC)成熟及TLR-4表达的影响,探讨蛋氨酸脑啡肽对小鼠骨髓来源DCs免疫功能的调节机制。体外制备小鼠骨髓细胞用GM-CSF协同MENK诱导培养7d后用RT-PCR法检测TLR-4的mRNA表达,用western-blot检测TLR-4蛋白的表达,同时用流式细胞仪(FACS)分析DC表型特征,混合淋巴细胞反应(MLR)检测DC的功能。结果显示MENK协同诱导组树突状细胞TLR-4和共刺激分子的表达高于正常对照组(P<0.01)和GM-CSF诱导组(P<0.05)并具有强的激发MLR的能力。提示MENK可以促进DC成熟及TLR-4的表达,增强DC免疫功能。
To investigate the effect of methionine enkephalin (MENK) on the maturation and TLR-4 expression of mouse bone marrow-derived dendritic cells induced by macrophage-granulocyte colony-stimulating factor (GM-CSF) Regulatory mechanism of mouse bone marrow-derived DCs immune function. The mouse bone marrow cells were prepared in vitro by GM-CSF and MENK for 7 days. The mRNA expression of TLR-4 was detected by RT-PCR. The expression of TLR-4 protein was detected by western-blot. Analysis of DC phenotype characteristics, mixed lymphocyte reaction (MLR) detection of DC function. The results showed that the expression of TLR-4 and costimulatory molecules in dendritic cells induced by MENK was higher than that in normal control group (P <0.01) and GM-CSF induction group (P <0.05) and had a strong ability to stimulate MLR. Prompted that MENK can promote DC maturation and TLR-4 expression, enhance DC immune function.