以蛇苔配子体为材料,以Prat和MS为基本培养基,初步研究了蛇苔组织培养的基本条件。结果表明:在Prat培养基上蛇苔未经原丝体阶段直接长出配子体。在不添加任何生长调节物质的MS培养基上,仅加入3%~5%的葡萄糖,即可以诱导出蛇苔愈伤组织。蛇苔愈伤组织在MS+4%葡萄糖+1.0 mg/L 2,4-D上分化长出叶状蛇苔配子体。 Taking snake moss gametophyte as material and Prat and MS as basic culture medium, the basic conditions of culturing snake moss were studied. The results showed that the snake moss grew directly on the Prat medium without the precursor phase. On MS medium without adding any growth regulator, only 3% ~ 5% glucose was added to induce the moss callus. The snake moss callus differentiated to leaf-like snake moss gametophytes on MS + 4% glucose +1.0 mg / L 2,4-D.