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用相转移法制备了用于引导骨再生的纳米磷灰石/聚酰胺66(n-HA/PA66)和载银纳米羟基磷灰石/二氧化钛/聚酰胺66(Ag-HA-TiO2/PA66)复合生物材料屏障膜,并通过与骨髓基质干细胞(BMSC)共培养评价了其体外生物相容性。扫描电子显微镜(SEM)观察显示,制备的复合材料膜为不对称多孔膜,一面是孔径小于10μm的微孔层,另一面是孔径在30~200μm的大孔结构。四唑盐比色(MTT)和流式细胞术(FCM)试验结果表明:n-HA/PA66膜具有良好的细胞亲和力,有利于BMSC的黏附、生长和减少凋亡;Ag-HA-TiO2/PA66膜也具有良好的生物相容性,但加速了BMSC的凋亡。2种膜的结构和生物相容性能够满足引导骨组织再生膜材料的要求。
The nano-apatite / polyamide 66 (n-HA / PA66) and silver-loaded nano-hydroxyapatite / titania / polyamide 66 (Ag-HA-TiO2 / PA66) The composite biomaterial barrier membrane was evaluated for its in vitro biocompatibility by co-culture with bone marrow stromal stem cells (BMSC). Scanning electron microscopy (SEM) observation shows that the prepared composite membrane is an asymmetric porous membrane, one side is a microporous layer with a pore size of less than 10 μm, and the other side is a macroporous structure with a pore size of 30-200 μm. The results of MTT assay and flow cytometry (FCM) showed that n-HA / PA66 membrane had good cell affinity, which was beneficial to the adhesion, growth and apoptosis of BMSC. Ag- The PA66 membrane also has good biocompatibility, but accelerates BMSC apoptosis. The structure and biocompatibility of the two kinds of membranes can meet the requirements of guiding the regenerated membrane materials of bone tissue.