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目的:研究中药复方松友饮对姑息性肝切除裸鼠模型中残癌生长及转移的影响。方法:以人肝癌细胞(MHCC97H)建立裸鼠移植瘤姑息性肝切除模型,36只裸鼠随机分成对照组和松友饮组,每天1次灌胃给药连续5周,每周测体质量。给药结束后第2d每组处死6只裸鼠观察肿瘤大小、肺转移等;并检测残癌中白细胞介素-1β(IL-1β)和增殖细胞核抗原(PCNA)表达情况。剩余裸鼠用于观察生存期。结果:松友饮组与对照组残癌体积为(724.9±337.6)mm3和(1205.2±581.3)mm3(P<0.05),抑瘤率为39.9%;肺转移分级下降(P<0.05)。前者体质量大于对照组(P<0.001),生存期延长率为22.7%;IL-1βmRNA表达水平显著下调(P<0.001),蛋白水平为(18.0±5.1)pg/mL和(27.9±4.5)pg/mL(P<0.01);PCNA指数为(75.2±8.5)%和(86.1±4.8)%(P<0.001)。结论:松友饮灌胃给药下调残癌表达IL-1β和PCNA,抑制残癌生长、转移,对肝癌病人有潜在的应用价值。
Objective: To study the effect of Chinese medicine compound Songyou Yin on residual cancer growth and metastasis in palliative liver resection model in nude mice. Methods: The model of palliative hepatectomy was established in nude mice with human hepatoma cells (MHCC97H). Thirty-six nude mice were randomly divided into control group and Songyou Decoction group. The nude mice were given gavage once daily for 5 weeks and the body mass was measured weekly. Six nude mice were sacrificed on the second day after the administration to observe the tumor size and lung metastasis. The expression of interleukin-1β (IL-1β) and proliferating cell nuclear antigen (PCNA) in the residual carcinoma was detected. The remaining nude mice were used to observe survival. Results: The residual tumor volume of Songyou drink group and control group was (724.9 ± 337.6) mm3 and (1205.2 ± 581.3) mm3 respectively (P <0.05). The tumor inhibition rate was 39.9%. The lung metastasis grade was decreased (P <0.05). The body weight of the former was higher than that of the control group (P <0.001), the survival rate was 22.7%, the level of IL-1βmRNA was significantly decreased (P <0.001), the protein level was (18.0 ± 5.1) pg / mL and (27.9 ± 4.5) pg / mL (P <0.01). The PCNA index was (75.2 ± 8.5)% and (86.1 ± 4.8)% respectively (P <0.001). Conclusion: Gouyou Decoction can reduce the expression of IL-1β and PCNA in residual cancer and inhibit the growth and metastasis of residual cancer, which may have potential value in patients with liver cancer.