论文部分内容阅读
目的 :观察急性重症胆管炎 (ACST)大鼠肺组织内一氧化氮合酶 (NOS)、肺泡巨噬细胞分泌一氧化氮 (NO)的变化及活血清解灵的调节作用。 方法 :通过胆总管远端结扎 ,近端注入菌液并封闭的方法 ,造成大鼠ACST模型。采用单纯胆管减压、胆管减压配合活血清解灵两种不同方法治疗。分离和培养肺泡巨噬细胞 ,采用Griess法检测收集培养液中NO水平。应用分光光度法测定NOS活性。 结果 :ACST组肺内NOS的水平和肺泡巨噬细胞分泌NO的水平显著高于假手术对照组 (P <0 .0 5 ) ,中药活血清解灵治疗组亦高于假手术对照组 ,但明显低于ACST组 (P <0 .0 5 ) ,减压组与ACST组无差异 (P >0 .0 5 )。 结论 :活血清解灵具有调节NOS的作用 ,抑制肺泡巨噬细胞过度释放NO ,减少NO对组织细胞的损伤。
Objective : To observe the changes of nitric oxide synthase (NOS) and nitric oxide (NO) secreted by alveolar macrophages in the lung tissue of severe acute cholangitis (ACST) rats and the regulating effect of live serum solution. METHODS: The rat ACST model was induced by distal ligation of the common bile duct, proximal injection of the broth and closure. The use of simple biliary decompression, biliary decompression combined with live serum solution two different methods of treatment. Alveolar macrophages were isolated and cultured, and NO levels were measured by Griess method. Spectrophotometric method was used to measure NOS activity. RESULTS: The level of NOS in the lung and the level of NO secreted by alveolar macrophages in the ACST group were significantly higher than those in the sham-operated control group (P < 0.05), and the serum in the active blood serum was also higher than the sham-operated control group. Compared with ACST group (P <0.05), there was no difference between decompression group and ACST group (P > 0.05). Conclusion : Live blood serum solution can regulate NOS, inhibit the excessive release of NO from alveolar macrophages and reduce the damage of NO to tissue cells.