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目的:观察三七对单侧输尿管梗阻大鼠肾组织和尿中细胞间黏附分子-1(ICAM-1)表达的影响,初步探讨其肾脏保护作用的部分分子生物学机制。方法:采用单侧输尿管梗阻(UUO)诱导大鼠肾小管间质纤维化模型。将52只SD大鼠随机分为假手术组(n=12)、模型组(n=20)、三七0.84 g.kg-1.d-1组(n=20)。术后第7,14天留取标本处死大鼠,处死前24 h留取大鼠尿液用双抗体夹心酶联免疫吸附法(ELISA)检测ICAM-1的变化;用免疫组化方法检测肾组织内ICAM-1表达和定位。结果:与假手术组相比,模型组大鼠第7,14天肾小管间质损伤评分明显增高(P<0.05),肾组织及尿ICAM-1表达水平明显升高,且2者之间以及肾组织ICAM-1与肾小管间质损伤病理评分(肾损伤指数)呈正相关关系。三七0.84 g.kg-1.d-1组肾脏病理改变及肾组织和尿中ICAM-1的表达明显受到抑制(P﹤0.05);与同期模型组比较有明显统计学意义(P﹤0.05)。结论:UUO模型组大鼠肾组织ICAM-1表达与肾损伤指数和尿ICAM-1水平分别呈正相关,提示尿中ICAM-1可作为检测肾组织ICAM-1的表达水平的指标。三七改善了模型组大鼠肾小管间质的病理组织学改变,对肾小管间质纤维化具有一定的保护作用,其机制可能部分地与下调肾组织和尿中ICAM-1的水平有关。
OBJECTIVE: To observe the effect of Panax notoginseng on the expression of intercellular adhesion molecule-1 (ICAM-1) in renal tissues and urine of rats with unilateral ureteral obstruction, and to explore the molecular mechanism of renal protection. METHODS: Unilateral ureteral obstruction (UUO) was used to induce rat tubulointerstitial fibrosis model. Fifty-two SD rats were randomly divided into sham operation group (n=12), model group (n=20) and Panax notoginseng 0.84 g.kg-1.d-1 group (n=20). The rats were sacrificed on the 7th and 14th day after surgery. The urine of the rats was taken 24 h before the death. The changes of ICAM-1 were detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA); the kidneys were detected by immunohistochemistry. Intracellular ICAM-1 expression and localization. RESULTS: Compared with the sham-operated group, the scores of renal tubulointerstitial injury on the 7th and 14th day in the model group were significantly higher (P<0.05), and the expression of ICAM-1 in the renal tissue and urine was significantly increased. And there was a positive correlation between renal tissue ICAM-1 and pathological scores of renal tubulointerstitial injury (kidney injury index). The renal pathological changes and the expression of ICAM-1 in renal tissues and urine of Panax notoginseng 0.84 g.kg-1.d-1 group were significantly inhibited (P<0.05), and there was significant statistical significance compared with the same period model group (P<0.05). ). Conclusion: The expression of ICAM-1 in renal tissue of UUO model rats was positively correlated with the renal injury index and urinary ICAM-1 levels, suggesting that ICAM-1 in urine can be used as an indicator to detect the expression level of ICAM-1 in renal tissue. Notoginseng improved the pathological and histological changes of tubulointerstitium in model rats, and had a protective effect on tubulointerstitial fibrosis. The mechanism may be partly related to the down-regulation of ICAM-1 levels in renal tissue and urine.