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目的探讨高浓度氧致新生鼠视网膜细胞损伤及雌激素干预的意义。方法建立新生wistar大鼠高氧诱导视网膜损伤模型,分为对照组、高氧组和高氧+雌激素组各20只。采用常规病理切片观察视网膜细胞的变化及组织结构变化;TUNEL染色观察视网膜细胞凋亡情况;RT-PCR检测谷氨酰胺合成酶(GS)mRNA的表达。结果对照组组织形态、细胞凋亡数目及GSmRNA表达均无明显变化。高氧组第6、7天细胞明显水肿、变形、崩解、结构紊乱,第8、10天细胞数目减少,层次变薄;细胞凋亡率第7天达高峰(2.13±0.66),第10天下降至(0.74±0.29),均高于对照组(P<0.01);GSmRNA表达第6天明显升高(0.62±0.05),第7天降至正常水平以下(0.46±0.06)。高氧+雌激素组细胞变化无高氧组明显;细胞凋亡率明显低于高氧组(P<0.05);GSmRNA表达第6天明显升高,第7天达高峰(0.80±0.06),高于高氧组(P<0.01)。结论高氧诱导GSmRNA抑制,引起视网膜细胞凋亡性损伤。雌激素可以改善视网膜细胞存在的内环境,从而达到有效保护视神经细胞的目的。
Objective To investigate the significance of retinal cell injury and estrogen intervention induced by hyperoxic oxygen in neonatal rats. Methods The model of retinal damage induced by hyperoxia in neonatal wistar rats was established and divided into control group, hyperoxia group and hyperoxia + estrogen group. The changes of retinal cells and histological changes were observed by routine pathological sections. The apoptosis of retinal cells was observed by TUNEL staining. The expression of glutamine synthetase (GS) mRNA was detected by RT-PCR. Results There was no significant change in the morphology of the control group, the number of apoptotic cells and the expression of GS mRNA. At 6 and 7 days, the cells in the hyperoxia group were obviously edema, deforming, disintegrating and disorganized. The numbers of the cells decreased and the layers became thinner on the 8th and 10th day. The apoptosis rate reached the peak on the 7th day (2.13 ± 0.66) (0.74 ± 0.29), all of which were higher than that of the control group (P <0.01). GS mRNA expression increased significantly (0.62 ± 0.05) on the 6th day and decreased to 0.46 ± 0.06 on the 7th day. The cells in hyperoxia + estrogen group had no significant changes in hyperoxia group and the apoptosis rate was significantly lower than that in hyperoxia group (P <0.05). GS mRNA expression increased significantly on the 6th day and reached the peak on the 7th day (0.80 ± 0.06) Higher than the hyperoxia group (P <0.01). Conclusion Hyperoxia induces the inhibition of GS mRNA and causes apoptosis of retinal cells. Estrogen can improve the inner environment of retinal cells, so as to achieve the purpose of effectively protecting optic nerve cells.