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以旷场试验法测定动物在急、慢性躯体性和心理性应激时的行为变化，以快速断头冷冻匀浆法，用ＨＩＴＡＣＨＩ－８３５氨基酸分析仪测定应激各期视皮层、海马、下丘脑、小脑谷氨酸含量。并对正常动物经侧脑室微量注射Ｌ－ＡＰ４后观察行为变化。结果显示，急性应激期动物行为活动增加，慢性应激期减少：应激时，大鼠部分脑区的谷氨酸含量与对照组相比在不同时期呈显著性差异；侧脑室微量注射Ｌ－ＡＰ４提示行为活动减弱可能与脑内Ｇｌｕ系统的活动有关。
The open field test was used to determine the behavioral changes of the animals under acute and chronic somatic and psychological stress. Rapid freezing was used to homogenize the frozen sections of the visual cortex, hippocampus and hippocampus in each stress period by HITACHI-835 amino acid analyzer Thalamus, cerebellar glutamate content. The behavioral changes of normal animals were observed after microinjection of L-AP4 into lateral ventricle. The results showed that during acute stress period, animal behavior increased and chronic stress decreased: In stress, the content of glutamate in some brain regions of rats was significantly different from that in control group at different time; -AP4 suggests that weakened behavioral activity may be related to the activity of the Glu system in the brain.