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目的 研究红细胞、淋巴细胞和粒细胞分别在枸橼酸钠抗凝自身血浆中对肿瘤细胞的作用 ,并初步探讨其机理。方法 5 0 μl 10 8/ml的红细胞悬液、10 7/ml淋巴细胞或 10 7/ml粒细胞悬液与等体积新鲜枸橼酸钠抗凝自身血浆和 10 0 μl的 10 6/ml的肿瘤细胞悬液混合 ,37℃ ,30min ,显微镜下观察红细胞、淋巴细胞和粒细胞对肿瘤细胞的粘附情况 ;设立抗CR1分子单抗阻断实验、依地酸钠(EDTA)抗凝自身血浆替代实验以及血浆天然抗体测定等进行机理探讨。结果 红细胞、淋巴细胞和粒细胞在自身血浆中对肿瘤细胞都有较强的免疫粘附作用。正常人红细胞、淋巴细胞和粒细胞的免疫粘附结合率分别为 6 4.1± 12 .5、5 8.7± 9.1和 6 0 .7± 10 .2 ,其中红细胞最早粘附结合肿瘤细胞。正常人群、肝癌及风湿性疾病患者的红细胞、淋巴细胞和粒细胞对肿瘤细胞的免疫粘附能力存在明显差异 (P <0 .0 1)。抗CR1分子单抗阻断或EDTA抗凝血浆替代实验都可阻断红细胞对肿瘤细胞的免疫粘附 ,而淋巴细胞和粒细胞的粘附作用只有部分被阻断。肿瘤细胞系通过替代途径激活补体。结论红细胞、淋巴细胞和粒细胞在自身血浆中对肿瘤细胞都具有较强的免疫粘附作用。红细胞的免疫粘附作用属补体依赖性CR1分子介导的天然免疫粘附。红细胞可明显促?
Objective To study the effects of red blood cells, lymphocytes and granulocytes on the antitumor autologous plasma of sodium citrate, and to explore the mechanism. Method 50 μl 10 8 /ml of red cell suspension, 10 7 /ml lymphocytes or 10 7 /ml granular cell suspension with equal volume of fresh sodium citrate anticoagulated autologous plasma and 100 μl of 10 6 /ml The tumor cell suspension was mixed at 37°C for 30 minutes and the adhesion of red blood cells, lymphocytes, and granulocytes to tumor cells was observed under a microscope; an anti-CR1 monoclonal antibody blocking experiment was established, and sodium edetate (EDTA) anticoagulated autologous plasma was established. Substitution experiments and determination of plasma natural antibodies were performed to investigate the mechanism. RESULTS Red blood cells, lymphocytes, and granulocytes have a strong immunoadhesion effect on tumor cells in their own plasma. The ratio of immune adherence of normal human red blood cells, lymphocytes, and granulocytes was 64.1±12.5, 58.7±9.1, and 60.7±10.2, respectively, and the earliest adhesion and binding of red blood cells to tumor cells. The erythrocytes, lymphocytes, and granulocytes of the normal population, liver cancer, and rheumatic diseases had significant differences in the immune adherence of tumor cells (P < 0.01). Anti-CR1 monoclonal antibody blocking or EDTA anticoagulant plasma replacement experiments can block the red blood cells from adhering to tumor cells, while the adhesion of lymphocytes and granulocytes is only partially blocked. The tumor cell line activates complement through an alternative pathway. Conclusion Red blood cells, lymphocytes and granulocytes have strong immunoadhesion to tumor cells in their own plasma. The immunoadhesion of red blood cells is a complement-dependent CR1 molecule mediated innate immune adhesion. Red blood cells can significantly promote?