论文部分内容阅读
棉花纤维发育过程包括起始、伸长、次生壁加厚和脱水成熟等阶段,是一个较为复杂的网络基因调控过程。为分析相关基因在不同棉种(属)纤维发育过程中时空表达特性,本研究以陆地棉、海岛棉和亚洲棉为研究对象,选取已确定与棉纤维发育相关的特异基因(GhACT1,GhCAD1,GhCCR1,GhExp1,GhExp2,GhCIPK1和GhPL),利用实时荧光定量PCR技术,系统分析这些基因在棉纤维发育过程的授粉当天(0 day post anthesis,0 DPA)、5天(5 DPA)、10天(10 DPA)、15天(15 DPA)、20天(20 DPA)、25天(25 DPA)的表达趋势变化。研究结果表明,所选特异基因在棉花纤维发育过程都有较高表达水平,但由于遗传背景不同,这些基因在不同棉种(属)纤维发育过程中表达趋势呈一定差异。通过对比分析GhExp1和GhExp2的基因表达趋势,本研究推测GhExp1对棉花纤维伸长的作用效果较为显著。此外,考虑到陆地棉、海岛棉和亚洲棉在纤维长度等方面的差异性,本研究推测GhACT1和GhExp1表达趋势变化对调控棉花纤维伸长有着重要作用。本研究结果可为深入分析棉花纤维发育相关基因与棉花纤维伸长的相关性提供参考。
Cotton fiber development, including the initial, elongation, secondary wall thickening and dehydration stages of maturation, is a more complex network of gene regulation process. In order to analyze the spatiotemporal expression characteristics of related genes in the development of different cotton (Genus) species, the effects of GhACT1, GhCAD1, GhCCR1, GhExp1, GhExp2, GhCIPK1 and GhPL) were used to analyze the expression of these genes during cotton fiber development at 0 DPA, 5 days (5 DPA) and 10 days 10 DPA), 15 days (15 DPA), 20 days (20 DPA), 25 days (25 DPA). The results showed that the specific genes selected had higher expression level in cotton fiber development. However, due to the different genetic backgrounds, the expression trends of these genes in different cotton (fiber) development were different. Through comparative analysis of GhExp1 and GhExp2 gene expression trends, the present study speculated that GhExp1 cotton fiber elongation effect is more significant. In addition, taking into account the differences in the fiber length of Gossypium hirsutum, Gossypium barbadense and Asiatic cotton, we speculated that the changes of GhACT1 and GhExp1 expressions play an important role in the regulation of cotton fiber elongation. The results of this study provide a reference for further analysis of the correlation between cotton fiber development related genes and cotton fiber elongation.