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应用高盐溶液提取法，高速离心分离纯化创伤小鼠脾细胞核蛋白，其核提取物经蛋白电泳分析，可见清晰蛋白条带。凝胶滞留法检测ＮＦ－κＢ的ＤＮＡ结合活性灵敏可靠。该方法的建立，为研究基因转录调控的表达奠定了基础。
High salt solution extraction method was used to purify the splenocyte nucleoprotein of traumatic mouse by high speed centrifugation. The nuclear extract was analyzed by protein electrophoresis, showing a clear protein band. Gel retention assay of DNA binding activity of NF-κB sensitive and reliable. The establishment of this method laid the foundation for the study of gene transcription regulation.