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目的:制备将药物治疗与热疗相结合的靶向抗癌药物新剂型一司莫司汀磁性脂质体。方法:用改良化学沉淀法制备Fe3O4纳米磁性粒子,运用透射电镜和PE热分析系统进行表征,在高频交变磁场下进行体外加热试验。采用反相蒸发超声法加高速搅拌制备纳米司莫司汀磁性脂质体,用透射电镜、图像分析系统和能谱仪对其进行表征,HPLC法检验脂质体中司莫司汀的包封率。结果:Fe3O4纳米磁性粒子近似球形,粒径10~40 nm。居里温度在120℃~140℃之间.其磁流体在高频交变磁场下可升温至35℃~47℃并保持恒定。以此磁性材料为载体制成的司莫司汀磁性脂质体的平均粒径为184.5nm,其中含有Fe3O4成份,药物包封率达到73.72%。结论:Fe3O4纳米粒子是制备医用磁性脂质体的良好载体,采用反相蒸发超声法加高速搅拌可制备纳米级Fe3O4磁性脂质体。
OBJECTIVE: To prepare a novel liposomal microstomatide liposome which combines the drug therapy with hyperthermia. Methods: Fe3O4 nano-magnetic particles were prepared by modified chemical precipitation method, characterized by transmission electron microscopy and PE thermal analysis system, and heated in vitro under high frequency alternating magnetic field. The nanostermostatin magnetic liposomes were prepared by reverse phase ultrasonication and high speed stirring. The magnetic liposomes were characterized by transmission electron microscopy, image analysis system and energy dispersive spectrometer. The encapsulation of semustine in liposomes was tested by HPLC rate. Results: The Fe3O4 nano-magnetic particles are spherical and have a size of 10-40 nm. The Curie temperature is between 120 ° C. and 140 ° C. The magnetic fluid can be heated to 35 ° C.-47 ° C. in a high-frequency alternating magnetic field and kept constant. The average particle size of the semustine magnetic liposome made of the magnetic material as a carrier was 184.5 nm, which contained Fe3O4 and the drug entrapment efficiency reached 73.72%. Conclusion: Fe3O4 nanoparticle is a good carrier for the preparation of medical magnetic liposomes. The nanoscale Fe3O4 magnetic liposomes can be prepared by reverse phase ultrasonication with high speed stirring.