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目的应用生物发光成像技术,非侵入性地连续检测活体裸鼠原位和异位脑肿瘤发展演进过程。方法用SMPU-R-MND-luc载体转染人脑肿瘤U87MG细胞系,形成具有高荧光素酶活性的细胞克隆。在裸鼠脑内和胁腰部皮下植入持续表达荧光素酶的肿瘤细胞,建立原位和异位脑肿瘤模型,用影像学资料显示肿瘤部位。用光子发射定量分析动态监测肿瘤生长情况。结果成功地建立了表达荧光素酶活性的原位和异位脑肿瘤动物模型。采集反映肿瘤生长的生物发光信号,肿瘤细胞植入后不同时间点的发光信号值呈显著正相关,而且原位和异位脑肿瘤间存在明显差异。但生物发光脑肿瘤生物发光信号值在第4 d和第14 d时无显著差异。结论体内生物发光成像可以非侵入性地动态检测活体内脑肿瘤演进过程,为研究肿瘤发展机制及最佳治疗策略的选择提供了新的手段和工具。
Objective To evaluate the development and progression of orthotopic and ectopic brain tumors in nude mice by noninvasive biofluorescence imaging. Methods The human brain tumor U87MG cell line was transfected with SMPU-R-MND-luc vector to form a cell clone with high luciferase activity. The nude mice were implanted subcutaneously with tumor cells expressing luciferase continuously in the flank and in the flank of the lumbar spine. Both in situ and ectopic brain tumor models were established and the tumor sites were visualized with imaging data. Quantitative dynamic analysis of tumor growth by photon emission. Results The animal model of in situ and ectopic brain tumors expressing luciferase activity was established successfully. The bioluminescence signals reflecting tumor growth were collected, the luminescence signal values at different time points after tumor cell implantation were significantly and positively correlated, and there was a significant difference between the eutopic and ectopic brain tumors. However, there was no significant difference in bioluminescent brain tumor bioluminescence signal at day 4 and day 14. Conclusion In vivo bioluminescence imaging can noninvasively and dynamically detect the evolution of brain tumors in vivo and provide new tools and tools for studying the mechanisms of tumor development and the optimal treatment strategies.