目的报道了功能性L-Cys-CdS纳米荧光探针的合成,纳米粒子水溶性好,稳定,且具有较好的荧光特性。方法以该纳米粒子为荧光探针,探讨了DNA对其的荧光猝灭作用。结果表明在Tris-HCL溶液中DNA对CdS的RLS有很好的猝灭,小牛胸腺DNA及鱼精DNA的线性范围及检测限分别为0.01 -1.0μg·mL-1, 8 ng·mL-1; 0.04 -1.5μg·mL-1, 10ng·mL-1。结论该方法简便、快速、灵敏,用于测定合成样品取得了满意的结果。 Objective To report the synthesis of functionalized L-Cys-CdS nanofluorescent probe, which has good water-solubility and stability, and has good fluorescence properties. Methods The nanoparticles were used as fluorescent probes to probe the fluorescence quenching effect of DNA. The results showed that the RLS of DNA in CdS was quenched well in Tris-HCL solution. The linear range and the detection limit of DNA in calf thymus DNA and fish sperm DNA were 0.01-1.0μg · mL-1 and 8 ng · mL- 1; 0.04-1.5μg · mL-1, 10ng · mL-1. Conclusion The method is simple, rapid and sensitive and has been used to determine the synthetic samples with satisfactory results.