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目的:建立HPLC-ELSD法同时测定消栓通胶囊中三七皂苷R1和人参皂苷Rg_1含量的方法。方法:色谱柱为Phenomenex kromasil C18(150mm×4.6mm,5μm),流动相:乙腈(A)-水(B)梯度洗脱程序:0~10min 90%B,10~15min 75%B,15~20min 50%B,20~25min 50%B,流速为1.0mL·min~(-1)。ELSD参数设定:气体流速为2.3L·min~(-1),漂移管温度为105℃。结果:三七皂苷R1和人参皂苷Rg_1分别在15.28~152.8μg·mL~(-1)(r=0.999 2)和49.62~496.2μg·mL~(-1)(r=0.999 0)范围内呈良好的线性关系,平均回收率分别为96.8%、98.0%,RSD分别为1.7%、2.1%(n=6)。结论:建立HPLC-ELSD法同时测定消栓通胶囊中三七皂苷R1、人参皂苷Rg_1含量方法专属性高,精密度、重现性良好,可用于消栓通胶囊的质量控制。
OBJECTIVE: To establish a HPLC-ELSD method for the simultaneous determination of notoginsenoside R1 and ginsenoside Rg_1 in Xiao Changtong capsules. Methods: The gradient elution was performed on a Phenomenex kromasil C18 column (150 mm × 4.6 mm, 5 μm) using a mobile phase of acetonitrile-water (B) gradient: 90% B for 0-10 min, 75% 20min 50% B, 20 ~ 25min 50% B, the flow rate of 1.0mL · min ~ (-1). ELSD parameters set: gas flow rate of 2.3L · min -1, drift tube temperature of 105 ℃. Results: Notoginsenoside R1 and ginsenoside Rg_1 were in the range of 15.28-152.8 μg · mL -1 (r = 0.999 2) and 49.62-496.2 μg · mL -1 (r = 0.999 0) respectively Good linear relationship, the average recovery rates were 96.8%, 98.0%, respectively, RSD 1.7%, 2.1% (n = 6). Conclusion: The HPLC-ELSD method for simultaneous determination of notoginseng saponins R1, ginsenoside Rg_1 content of specificity, precision, reproducibility, and can be used for the quality control of Xiao Changtong capsule.