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目的 :了解胰岛素样生长因子1 (IGF1 )对体外生长的兔关节软骨细胞增殖以及细胞超微结构的影响。方法 :应用兔关节软骨细胞体外单层培养 ,对照组培养液为含 1 0 %小牛血清DMEM ,实验组在培养液DMEM中加入IGFl使其终浓度为 1 0 0 μg·L- 1 ,作用细胞 1周 ,得出细胞生长曲线 ,并分别测得两组DNA含量。同时观察IGFl 作用后 ,软骨细胞超微结构的变化。结果 :结果显示IGFl能明显促进培养软骨细胞增殖。实验组DNA含量 ,与对照组相比 ,有统计学意义 (P<0 .0 1 )。在电镜下 ,细胞主要表现为增殖性改变 ,说明细胞代谢活跃。结论 :IGFl 可刺激软骨细胞增殖 ,细胞超微结构表现代谢活跃状态
Objective: To investigate the effect of insulin-like growth factor 1 (IGF-1) on the proliferation and ultrastructure of rabbit articular chondrocytes cultured in vitro. Methods: Rabbit articular chondrocytes were cultured monolayer in vitro. The control group contained 10% fetal bovine serum (DMEM). In the experimental group, IGF1 was added to culture medium DMEM so that the final concentration was 100 μg · L -1 After 1 week, the cell growth curve was obtained, and the DNA content of the two groups were measured respectively. At the same time observe the changes of the ultrastructure of chondrocytes after the action of IGF1. Results: The results showed that IGF1 can significantly promote the proliferation of cultured chondrocytes. The DNA content of the experimental group, compared with the control group, was statistically significant (P <0.01). Under electron microscopy, the cells showed mainly proliferative changes, indicating that cell metabolism is active. Conclusion: IGF1 can stimulate the proliferation of chondrocytes, the ultrastructure of cells showed metabolic activity