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目的:探讨磷酸化钙调蛋白依赖性激酶Ⅱ(pCaMKⅡ)在异氟烷吸入全身麻醉作用中的作用及相关机制。方法:采用经侧脑室置管全脑给药,将SD大鼠随机分为溶剂对照组(control组)和CaMKⅡ抑制剂十四烷酰肉豆蔻酰-钙调蛋白自变性抑制肽(myrAIP)处理组(n=10),给药40min后行异氟烷吸入麻醉(1MAC,30min),监测动物脑电,取脑电波平稳清晰的波段,在异氟烷麻醉30min时间点进行分析,并于异氟烷麻醉30min取脑组织样本进行蛋白定量分析。结果:抑制剂组动物给药30min时脑电抑制波明显多于对照组(P<0.05);与对照组相比,抑制剂组脑组织pCaMKⅡ蛋白表达量明显降低(P<0.05)。结论:CaMKⅡ抑制剂能明显改变异氟烷全麻过程中大鼠的脑电变化及磷酸化蛋白表达变化,pCaMKⅡα参与了异氟烷全身麻醉的过程。
Objective: To investigate the role of phosphorylated calmodulin-dependent kinase Ⅱ (pCaMKⅡ) in general anesthesia induced by isoflurane inhalation and its related mechanism. Methods: The SD rats were randomly divided into control group (control group) and myrAIP (myristoyl myristoyl-calmodulin) inhibitory peptide (CaMKⅡ inhibitor) (N = 10). After isoflurane inhalation anesthesia (1MAC, 30min) was given 40min after administration, the EEG was monitored, and the EEG wave band was stable and clear.After 30min isoflurane anesthesia, Anesthesia 30 minutes after halothane brain samples for protein quantitative analysis. Results: Compared with the control group, the EEG wave amplitude in the inhibitor group was significantly higher than that in the control group at 30 min (P <0.05). Compared with the control group, the expression of pCaMK Ⅱ protein in the inhibitor group was significantly decreased (P <0.05). CONCLUSION: CaMKⅡ inhibitor can significantly change the changes of EEG and phosphorylation in rat during general anesthesia of isoflurane, and pCaMKⅡα participates in the process of general anesthesia of isoflurane.